Building on previous parabolic flights, the Human Tended Space Biology: Enabling Suborbital Genomics and Gene Expression suborbital flight test effort aims to develop operational concepts and deployment tests for gene expression analyses. Using plants as the test organisms, the project will conduct the first suborbital, human-tended, whole genome gene expression experiments. The tests will utilize fixation tubes from NASA’s Kennedy Space Center for safety containment and operational effectiveness, as well as fluorescent imaging hardware from the University of Florida, to measure the biological response of plants to gravity change.

Problem Statement 

This area of science, adaptation processes in response to transitional g loads to and from microgravity, is completely unknown and unapproached. This situation leaves a large gap in knowledge regarding the changes that occur in biology as organisms adapt to and from microgravity. That gap in knowledge can be richly filled using suborbital vehicles, and can best be filled using tools and technologies that can be deployed by humans within the vehicle, thereby keeping the experiment operations similar to earth bound and ISS experiments.

The technology of the KFTs has been well developed for many spaceflight and spaceflight related operational environments, however they have never been deployed in suborbital vehicles nor have the associated operations plans or associated facilities been developed for suborbital deployments.
Technology Maturation The technology development goal is to examine and test the effectiveness and limitations of deploying KFTs within commercial suborbital vehicles and during typical suborbital flight profiles. The scientific goal is to capture gene expression data from plants at various stages of the flight in order to characterize the rapid and early phases of the adaptation processes that accompany transitions among g-loads.

Summary of Flight Test
2024-08-29 This flight was able to demonstrate the development of research, hardware, and operations for a scientist astronaut to fly their science in space. The ability for a scientist to operate the experiment that was developed into study spaceflight effects of biology, is uniquely important, as the experience that a scientist and the biology they are studying are similar. By interpreting the conditions that the plants experienced, beyond what is recordable by a machine/data logger, will give the scientistastronaut unparalleled insight into the results.

• Innovative: First measurement of its kind during sub-orbital flight
• Focused: Research looks at the effect of multiple gravity environments
• Risk-reducing: Optimizes human health in high-altitude and suborbital flight 

Future Customers
• Human space exploration and long-duration crewed missions
• Biological research
• Material sciences
• Fluid physics