Description: There is an extraordinary need for a universal biomedical analyzer that has broadly flexible capabilities for cell studies, small animal experiments, and crew member health. The goal of our rHEALTH X device is to create a single palm-sized device with tripartite capabilities: non-invasive measurements, cell cytometry analysis, and multiplexed nanostrip tests. Currently, there is no single device that is able to provide comprehensive non-invasive measurements, let alone combine it with the rHEALTH's significant wet laboratory analytical capabilities. We have developed the existing rHEALTH technology in collaboration with NASA and here, in this Phase I proposal, we intend to further push the envelope and add a fully non-invasive module. The module will include measurements of heart rate, SpO2, body temperature, respiratory rate, and EKG. The module will be housed in the back of the rHEALTH X and will be fully detachable for wireless/wearable applications. At the end of Phase I, we will develop a TRL 4 non-invasive module. For Phase II, we will integrate the module with our existing rHEALTH technology into the rHEALTH X. We will bring it to TRL 7, so that it can be flight-certified and flown on the International Space Station (ISS) in a timely manner.

Benefits: SCIENCE APPLICATIONS: non-invasive measurements of cells and small animals, temperature monitoring of experiments, 4-parameter (2 fluorescence, side scatter, and forward scatter) flow cytometry applications, multiplexed gene expression analysis using nanostrips, biomarker measurements of cell changes, flexible assay capability for space research community, use of rHEALTH X in Microgravity Sciences Glovebox or Disposable Glove Bag to solve sample transfer issue. CREW HEALTH APPLICATIONS: real-time monitoring of astronaut health status, heart rate monitoring to detect arrhythmias, temperature to assess infection or fever, respiratory rate to determine breathing capability, EKG to measure rate and rhythm disturbances, SpO2 to measure respiratory function, measurement of overall physiological function, study of bone health via 25-OH vitamin D, assessment of immune function via TNFα, study of space radiation effects, systematic monitoring of astronaut health status during trainings, acute determination of trauma and blood loss.

POINT-OF-CARE DIAGNOSTICS: real-time health monitoring, real-time intervention, measurement of leukocytes to diagnose infection and inflammation, portable EKG to allow immediate measurement of heart rate and rhythms, temperature to assess infection and fever, respiratory rate to measure breath capability, SpO2 to measure oxygenation and breathing changes, acute determination of trauma and blood loss, comprehensive portable health monitor with tripartite capabilities, healthcare diagnosis at home and in the field, consumer health management.